The Culpepper Ensign (often referred to in error as a "Navy Jack")
Subsequently, there was a Naval rendition of the Culpepper Minutemen Flag. This was one of the first flags to show 13 stripes, one for each American colony, along with the rattlesnake (although some sources say that this was a poisonous sea snake). It features the snake above the warning, Don't Tread On Me. As previously mentioned, the rattle snake had become a traditional symbol of the American Colonies and the Colonial Navy apparently adopted the sea going version of this and the Culpepper name stuck with it.

This flag is believed to have flown aboard the Continental Fleet's flagship Alfred, in January, 1776. This flag or one of it's variations was used by American ships throughout the Revolution.